Microfluidic detection using G-quadruplex DNA-based biosensors
Year: 2024
Type: article
Abstract: The G-quadruplex is a noncanonical secondary structure formed by guanine-rich nucleic acid sequences stabilized by metal ions . Multiple types of human health related target detection strategies have been developed based on the G-quadruplex motif, owing to its high structure stability and manipulability. These G-quadruplex DNA based biosensors exhibit high specificity and sensitivity in a variety of detection strategies. In recent years, microfluidic-based detection platforms are pushing the development of molecular detection forward due to their advantages of high-throughput, high integration, low reagent and time consumption, and portability. By integrating the strengths of both G-quadruplex DNA and microfluidics, G-quadruplex-based microfluidic biosensors are emerging as one of the cutting-edge research approaches. This review provides an overview of the detection strategies of G-quadruplex DNA-based biosensors and the classification of microfluidic technologies. It then focuses on the application of G-quadruplex-based microfluidics in the detection of human health-related ions and biomarkers, as well as its innovative applications as drug screening tools. At the end of this review, we discuss the advantages and current challenges of G-quadruplex-based microfluidic detection platforms.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,000
Funders: National Natural Science Foundation of China, Science and Technology Development Fund, Universidade de Macau, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, National Key Research and Development Program of China, Fundamental Research Funds for the Central Universities, Key Research and Development Projects of Shaanxi Province, Basic and Applied Basic Research Foundation of Guangdong Province
OpenAlex ID: W4405825792