Antibacterial and Anticancer Properties of Diketopiperazines from Streptomyces antimicrobicus BN122, an Endophyte in Oryza sativa var. glutinosa
Year: 2024
Type: article
Abstract: Background and Objectives: This study characterized a bacterial strain, BN122, isolated from the root tissues of purple sticky rice (Oryza sativa L. var. glutinosa). Identified as Streptomyces antimicrobicus based on 16S rDNA analysis and physical-chemical properties, the aim was to isolate and evaluate the antibacterial and anticancer activities of its bioactive compounds. Materials and Methods: The major compounds were purified from BN122's culture extract using column chromatography and TLC. The NMR spectroscopy and mass spectrometry confirmed their identities as Cyclo-(L-Pro-L-Val), Cyclo-(L-Pro-L-Leu), Cyclo-(L-Pro-L-Trp) and Cyclo-(L-Pro-L-Phe). The antibacterial and anticancer activities of these compounds were subsequently assessed. Statistical significance was determined using SPSS software. Results: Isolated compounds exhibited potent antibacterial activity against Gram-positive bacteria. Minimum inhibitory concentrations (MICs) ranged from 32 to 256 μg/mL, while minimum bactericidal concentrations (MBCs) were between 128 and 512 μg/mL. Compounds demonstrated potent cytotoxic activity against cancer cells, with IC50 values ranging from 32.00 to 57.08 μg/mL for MDA-MB-231 cells, 85.73 to 158.93 μg/mL for HeLa cells and 276.89 to 323.48 μg/mL for HepG2 cells. Notably, these compounds exhibited moderate toxicity towards non-cancerous Vero cells (IC50 = 482.73 to 680.87 μg/mL). Conclusion: The findings suggested that Streptomyces antimicrobicus BN122 produces compounds with promising antibacterial and anticancer properties. Further research on these compounds could contribute to developing novel therapeutic strategies for bacterial infections and certain cancers.
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4405828510