Work

Development of the Antibody Response to a Vaccine during Growth of Juvenile Japanese Amberjack

Year: 2024

Type: article

Abstract: We investigated the antibody dynamics at different growth stages of Japanese amberjack Seriola quinqueradiata to determine the timing of first IgM production. Vaccination trials of juveniles were then conducted using a formalin-inactivated Vibrio anguillarum serotype J-O-3 vaccine to characterize the changes in vaccine-specific IgM and efficacy at each growth stage. In whole-body analyses, although total IgM was slightly detected at 1 day post-hatching (dph), it was undetectable until 15 dph and then increased from 23 to 57 dph. Levels of IgM specific to V. anguillarum J-O-3 were significantly elevated in fish vaccinated at and after 60 dph. Relative percentage survival of vaccinated fish was 52.6% and 100% at 51 dph and ≥61 dph, respectively. These results suggest that the IgM response to the vaccine developed between 51 and 60 dph (body weight: 0.45 ± 0.04 to 1.15 ± 0.06 g; standard length: 2.68 ± 0.09 to 3.90 ± 0.08 cm), coinciding with the increase in vaccine efficacy. These data also suggest that development of the humoral immunity was delayed from the initiation of IgM production.

Source: Fish Pathology

DOI: 10.3147/jsfp.59.127