Work

Generation of a USP9Y knockout human embryonic stem cell line with CRISPR-Cas9 technology

Year: 2024

Type: article

Abstract: Human embryonic stem cell (hESC) lines are vital tools for studying gene function, disease modeling, and therapy. We generated a USP9Y knockout hESC line using CRISPR-Cas9 in the male-derived H1 line. Targeted deletion of the USP9Y gene was confirmed via PCR and sequencing. The modified line retained pluripotency markers, exhibited a normal karyotype, and differentiated into all three germ layers. This model provides a valuable platform for studying USP9Y's role in human development and male infertility, offering insights into related disorders and therapeutic potential.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2024.103646

Authors: Sai Wei, Yuting Zhen, Chao Sun, Yanlin Ma, Qi Li, Luan Wen

Institution: Hainan Medical University

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,320