Whole Genome Sequence of Pseudomonas aeruginosa CIMT2, Isolated from the Rhizosphere of Maize (Zea mays L.)
Year: 2024
Type: article
Abstract: Sustainable agriculture aims to reduce the use of chemical fertilizers, with species from the Pseudomonas genus, a gram-negative bacterium, playing a key role as Plant Growth-Promoting Rhizobacteria (PGPR).These bacteria enhance nutrient uptake, support nitrogen metabolism, and improve plant tolerance to various biotic and abiotic stresses.In this study, we analyze P. aeruginosa strain CIMT2, isolated from the rhizosphere of maize (Zea mays L.).Pseudomonas exhibits halotolerance, phosphate solubilization, ammonia production, and multiple nitrogen metabolism activities, including nitrate reduction, indicating its potential as a PGPR, particularly in saline and nutrient-limited environments.By investigating its genetic composition, we aim to explore the contributions of P. aeruginosa CIMT2 to maize growth and productivity.Understanding the role of this strain in promoting plant health could offer a sustainable alternative to traditional agricultural practices, especially in stress-prone environments.The genome of P. aeruginosa strain CIMT2 was sequenced after its isolation on January 14, 2022, from the rhizosphere soil of maize.Approximately 1 g of rhizosphere soil was serially diluted up to 6-fold and spread onto tryptic soy agar (TSA) plates.The plates were incubated at 30℃ for 5 days.A single colony was selected, purified through repeated subculturing, and subsequently grown in tryptic soy broth (TSB) for 24 h to obtain sufficient biomass for molecular identification.Genomic DNA from P. aeruginosa strain was extracted using the Wizard Genomic DNA Purification Kit (Promega, USA) following the manufacturer's guidelines.DNA concentration was determined with a QubitThis study reports the whole genome sequence of Pseudomonas aeruginosa strain CIMT2, which was isolated from the rhizosphere soil of maize (Zea mays L.).The genome consists of 7.1 Mb with a total of 626,677,475 bp sequenced and the GC content is 65.8%.Notably, the genome contains key genes associated with plant growth-promoting activities, including those involved in nitrogen metabolism (e.g., ntrC, nosZ), phosphorus solubilization (e.g., pstS, phoB), and salt stress response (e.g., osmE, glnK).These traits may enhance the bacterium's role in promoting plant growth and soil health, underscoring its potential applications in sustainable agriculture.
Authors: Kanika Mahra, Tino Bashizi, Jae-Ho Shin
Institution: Kyungpook National University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4405871629