Peculiarities of humoral immunity parameters in newborns with markers of undifferentiated connective tissue dysplasia
Year: 2024
Type: article
Study design: Observational Study
Abstract: Connective tissue dysplasia can affect the immune response in children. The study of humoral immunity indicators in newborns with locomotor and biochemical markers of undifferentiated connective tissue dysplasia (UCTD) is very relevant. Aim - study the correlations between anthropometric, laboratory markers of UCTD and indicators of the B-cell link of immunity in newborns. Materials and methods. We examined 122 newborns: 79 full-term and 43 premature infants. In them, the perinatal history data were analyzed, anthropometric and biochemical (matrix metalloproteinase-1 (MMP-1), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1)) markers of UCTD were studied, and the ratio of MMP-1/TIMP-1 was calculated. The absolute number of B-lymphocytes and the levels of immunoglobulins of classes A (IgA), M (IgM), G (IgG) in the blood were also investigated. The main group (n=82) consisted of newborns with elevated values of ≥2 anthropometric markers of UCTD, the control group (n=40) included children without deviation from the norm of any anthropometric index. Results. Children with signs of UCTD are more likely to be born from ≥2 births, with a body weight of 2499-2000 g and ≤1999 g, and have lower levels of serum IgA, IgM than children of the control group. Such perinatal factors as operative delivery and parity of more than two births can be considered prognostically unfavorable for a lower absolute number of B-lymphocytes, lower levels of IgA and IgM in the blood of newborns. Serum content of MMP-1≥4.51 ng/ml, values of MMP-1/TIMP-1≥0.31 are associated with a higher absolute number of B-lymphocytes and lower level of IgA, also the concentration of TIMP-1≥16.08 ng/ml is associated with higher levels of IgA, IgM, IgG. The content of serum immunoglobulins correlates with the gestational age. Conclusions. Therefore, newborns with anthropometric markers of UCTD and an imbalance of biochemical markers (MMP-1, TIMP-1, index MMP-1/TIMP-1) have lower levels of serum IgA and IgM. The concentration of MMP-1≥4.51 ng/ml, MMP-1/TIMP-1≥0.31, TIMP-1≤16.07 ng/ml are prognostically unfavorable factors regarding changes in the B-cell immunity in newborns. The lowest concentrations of immunoglobulins of all classes were recorded in children with a gestational age of 31-28 weeks. The study was carried out according to the principles of the Declaration of Helsinki. The study protocol was accepted by the Local Ethical Committee of these institutions. The informed consent of the children's parents was obtained for the research. The authors declare no conflict of interest.
Source: Modern pediatrics Ukraine
Authors: O.Yu. Chumak, A.P. Volokha
Institution: Shupyk National Healthcare University of Ukraine
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4405876187