A comprehensive report on GC-MS profiling, FTIR analysis and HPLC quantification of pharmaceutically vital metabolite thymoquinone from Nigella seeds
Year: 2024
Type: article
Abstract: The present investigation aimed to gain insights into the structure of bioactive metabolic compounds in Nigella sativa L. seed oil. Initially, spectroscopic methods viz., GC-MS and FTIR were employed to determine functional groups, substituents, and conjugated double bonds in Nigella oil. GC-MS analysis identified 11 different amalgams, with p -cymene, ?-terpinene and ?-thujene being the major components. The FTIR spectrum revealed the presence of strong, sharp, and weak peaks, along with critical functional groups corresponding to C-H, O-H, C-C, C?N, and N-O, indicating the presence of pharmaceutically active constituents of the seed oil in the wavelength range of 400 – 4000 cm-1. HPLC analysis indicated that the percent composition of thymoquinone in the seed extract was reported as 0.90% at a wavelength of 254 nm. In the examined samples, thymoquinone and standard thymoquinone both showed a peak Rf value of 3.656. The study's findings revealed that thymoquinone is a potential phytochemical present in the oil. Furthermore, the identified biomolecules hold promise for use in pharmaceutical applications to enhance health standards.
Source: Plant Science Today
DOI: 10.14719/pst.4901
Authors: Y Ravi, P I Vethamoni, S N Saxena, N Ashoka, V Kavan Kumar, D Yallappa, P R Pavan, R Jeevitha, Dhamotharan P, S John, S K Bagra
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4405878674