Year: 2024
Type: article
Abstract: Aim. The aim of the study is to determine the features of the regeneration ability of explants of the species Prunus laurocerasus L. during in vitro cultivation, depending on the type of cutting and the phytohormonal composition of the nutrient media. Materials and methods. The study was conducted in the laboratory of microclonal propagation of the National Arboretum "Sofiyivka" of the NAS of Ukraine. The material for the study was non-lignified shoots of the species Prunus laurocerasus L., which were sterilized using 0.1% aqueous solution of HgCl2, 2% aqueous solution of mediocide and 75% solution of NaOCl, after which the plants were planted on hormone-free Murashige-Skoog nutrient media. Results. During the experiment, sterile explants were cultivated on hormone-free nutrient media, and within 6−10 days, the sterility and viability of the explants were determined, the most viable ones were selected and planted on nutrient media with different phytohormones. During the period of cultivation of the explants, their growth and development were monitored. The best morphometric indicators (number of shoots, length and reproduction coefficient) were found in explants cultivated on nutrient medium VI, which were 2.27±0.18; 2.12±0.14; 2.34±0.12, respectively. Conclusion. According to the results of the study, it was found that the highest percentage of sterility (83.27%) and viability (89.88%) were explants obtained from the medial part of the shoots after treatment with a 75% solution of sodium hypochlorite (NaOCl) with an exposure of six minutes. It was found that the highest regeneration ability (7.12±0.23) was possessed by explants obtained from the medial part of the shoot. On nutrient medium VI, modified by the addition of 0.22196 μm/l 6-BAP and 0.03936 μm/l β-IMC, the number of formed shoots was 6.95±0.19 pcs., and the length was 3.71±0.15 cm.
Source: Microbiology&Biotechnology
Authors: O. V. Pochka, L. A. Koldar
Institution: National Dendrological Park "Sofiyivka"
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4405886200