Figure 5 from Tumor Expression of CD83 Reduces Glioma Progression and Is Associated with Reduced Immunosuppression
Year: 2024
Type: other
Abstract: ALTs are sufficient to activate CD8+ T cells and act via cytokine release. In vitro tumor cells cocultured with isolated naïve CD8+ T cells (A). Multispectral imaging and flow cytometry for cocultured naïve CD8+ T cells demonstrating higher expression of mature CD3+CD8+ markers (B–E). ELISA for IFNγ showing increased in CD83OE cocultured T cells compared with control (two-way ANOVA: tumor type P value = 0.7105, treatment P value = 0.0021, interaction P value = 0.6425, Šidák MCT: tumor vs. treated tumor control P value = 0.1160, tumor vs. treated tumor control P value = 0.00236; F). ELISA for IL-2 in control and CD83OE tumors and cocultured T cells (two-way ANOVA: tumor type P value = 0.1314, treatment P value = 0.0232, interaction P value = 0.8087, Šidák MCT: tumor vs. treated tumor control P value = 0.2739, tumor vs. treated tumor control P value = 0.1437; G). ELISA for TNFα in control and CD83OE tumors and cocultured T cells (two-way ANOVA: tumor type P value = 0.8411, treatment P value = 0.9121, interaction P value = 0.3234; H). Increased expression of TNFα and IL-2 by CD83OE tumors compared with CD83KO and control tumors in scRNA-seq from pB-IUE tumors (I). *, P value < 0.05. (Created with Biorender.com.)
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4405886565