Work

Amphiphilic dynamic covalent polymer vectors of siRNA

Year: 2024

Type: article

Abstract: Dynamic covalent polymers (DCPs) recently emerged as smart siRNA delivery vectors, which dynamically self-assemble through siRNA templating and depolymerize in a controlled manner. Herein, we report the dynamic combinatorial screening of cationic and amphiphilic peptide-based monomers. We provide experimental evidence, by mass spectrometry analyses, of the siRNA-templated formation of DCPs, and show that amphiphilic DCPs display superior activity in terms of siRNA complexation and delivery in cells. Thus, the work describes a new type of siRNA vector based on dynamic covalent lipopolyplexes, which feature improved activity as well as better nano-structuration compared to previous generations of DCPs.

Source: Chemical Science

DOI: 10.1039/d4sc07668k

Authors: José García Coll, Pauline Trousselier, Sachin Dattram Pawar, Yannick Bessin, Laure Lichon, Jeanne Leblond Chain, Emmanuelle Sachon, Nadir Bettache, Sébastien Ulrich

Institutions: École Nationale Supérieure de Chimie de Montpellier, Centre National de la Recherche Scientifique, Université de Montpellier, Institut des Biomolécules Max Mousseron, Université Paris Sciences et Lettres, École Normale Supérieure - PSL, Sorbonne Université, Laboratoire des Biomolécules, Université de Bordeaux, Inserm, Acides nucléiques: Régulations naturelles et artificielles

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: Agence Nationale de la Recherche, Fondation ARC pour la Recherche sur le Cancer, Université de Montpellier, Sorbonne Université

Award: Selection and adaptation of siRNA vectors by live cells

OpenAlex ID: W4405887833

FWCI: 1.295

Cites: 39

Cited by: 11

Related to: 10