Year: 2024
Type: article
Abstract: Most bacteria lack membrane-enclosed organelles to compartmentalize cellular processes. In lieu of physical compartments, bacterial proteins are often recruited to macromolecular scaffolds at specific subcellular locations to carry out their functions. Consequently, the ability to modulate a protein’s subcellular location with high precision and speed bears the potential to manipulate its corresponding cellular functions. Here we demonstrate that the CRY2/CIB1 system from Arabidopsis thaliana can be used to rapidly direct proteins to different subcellular locations inside live E. coli cells including the nucleoid, the cell pole, membrane, and the midcell division plane. We further show that such light-induced re-localization can be used to rapidly inhibit cytokinesis in actively dividing E. coli cells and that the CRY2/CIBN binding kinetics can be modulated by green light, adding a new dimension of control to the system. Finally, we tested this optogenetic system in three additional bacterial organisms, B. subtilis, C. crescentus and S. pneumoniae, providing important considerations for the applicability of this system in microbiology and bacterial cell biology.
Source: Nature Communications
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,790
Awards: Local and global regulation of bacterial growth, Elucidating the spatiotemporal regulation of septal peptidoglycan synthases in E.coli, G1467953065, G1518161034, G1565140161, Program in Molecular Biophysics, G20932665, G3277370422, G3517702315, Spatial Organization and Regulation of Bacterial Cellular Processes, G3549828030, G3725638629, G3810702738, G3811920151, Probing the structure, dynamics and function of the E. coli divisome, Biochemistry, Cellular and Molecular Biology Program, G4884374849, New Regulatory Interactions and Circuits that Mediate the Dynamics, Homeostasis, and Stress Responses of Peptidoglycan Synthesis in the Superbug Streptococcus pneumoniae, G5520316217, G560005294, G5671063031, G6192103303, G676004083, G6958265442, G754529312, G7980756608, G8280635258, G8519948934, G9000030494, G9000038077, G9000054956, G9000236365
OpenAlex ID: W4405892438