Anti-inflammatory and anti-oxidant activities of Schumanniophyton magnificum leaves: In vivo and in vitro approaches
Year: 2024
Type: article
Abstract: Purpose: To investigate the methanol extract of Schumanniophyton magnificum leaves' (MESM-L) anti-inflammatory and antioxidant properties Methods: Acute toxicity studies were carried out for 24 hours. Albino rats were randomized into five groups for in vivo anti-inflammatory studies using three models namely egg albumin-induced paw edema, acetic acid-induced vascular permeability, and in vivo leukocyte mobilization assay. Groups include the control, standard and experimental groups (100, 200 and 400 mg/kg b.w. of MESM-L). Membrane stabilization and anti-platelet aggregatory response were carried out at the concentrations 0.1 – 0.8 mg/ml. Nitric oxide, 1, 1-diphenyl-2-picrylhydrazyl, and ferric-reducing antioxidant power assays were employed for anti-oxidant studies. Results: In acute toxicity investigations, MESM-L was found to be non-toxic at the maximum dose of 5000 mg/kg b.w. MESM-L (400 mg/kg b.w) inhibited edema by 73.13% and inhibited vascular permeability by 72.37%. A profound effect of MESM-L on leukocyte mobilization was detected at the lowest dose (41.17%). Inhibition of hemolysis and aggregation of platelets was exhibited with highest concentration of MESM-L on membrane stabilization and anti-platelet aggregatory. MESM-L scavenged NO and DPPH radical- with IC50 values of 19.52 and 12.31 μg/mL in that order. The effect of gallic acid on FRAP assay was found to be higher than MESM-L. Conclusion: These findings proved the anti-inflammatory potential of MESM-L, although the activities exhibited by the standard drugs were significantly higher. The capacity of the plant to scavenge free radicals is responsible for the effects that have been discovered.
DOI: 10.26538/tjdr/v1i1.2
Authors: Anosike Joy Chizoba, Ijoma Kingsley Ikechukwu, Aneke Rowland Jachike, Parker Elijah Joshua
Institutions: Godfrey Okoye University, University of Nigeria
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4405897218