Work

Combining Fourier Transform Ion Mobility with Charge Detection Mass Spectrometry for the Analysis of Multimeric Protein Complexes

Year: 2024

Type: article

Abstract: ) of individual ions, unlike conventional MS methods that use large ensembles of ions. CDMS typically requires long acquisition times and the collection of thousands of spectra, each containing tens to hundreds of ions, to generate sufficient ion statistics, making it difficult to interface with the time scales of online separation techniques such as ion mobility. Here, we demonstrate the application of Fourier transform multiplexing and drift tube ion mobility joined with Orbitrap-based CDMS for the analysis of multimeric protein complexes. Stepped frequency modulation was utilized to enable unambiguous frequency assignment during mobility sweeps and allow spectral averaging, which improves the accuracy and signal-to-noise of ion mobility spectra and CDMS measurements. Fourier transformation of the signal reveals the arrival times and collision cross sections of ions while simultaneously collecting charge information for thousands of individual ions. Combining Fourier transform multiplexing ion mobility and CDMS provides insight into each ion's size and mass while showcasing a potential solution to the duty cycle mismatch of online separation techniques in the single ion regime.

Source: Analytical Chemistry

DOI: 10.1021/acs.analchem.4c03379

Authors: Kyle J. Juetten, James D. Sanders, Michael T. Marty, Jennifer S. Brodbelt

Institutions: The University of Texas at Austin, University of Arizona

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, Welch Foundation, Division of Chemistry

Awards: Ultraviolet Photodissociation Mass Spectrometry, G3123266610, G4226750041, G6482141952, CAREER: Enabling nanodisc native mass spectrometry to study membrane active compounds