Year: 2024
Type: article
Abstract: This study examined the anti-proliferative and anti-migratory effects of a Grammatophyllum stapeliiflorum extract (GSE) on AGS gastric cancer cells, with a focus on its potential mechanisms against tumor growth and metastasis.Gastric cancer is a highly aggressive malignancy associated with a poor prognosis, necessitating novel therapeutic strategies to improve the outcomes.In this research, AGS cells were treated with various GSE concentrations, and the cell viability was evaluated using a WST-1 assay at multiple time points.The results revealed the dose-dependent inhibition of cell proliferation, with higher GSE concentrations leading to significantly reduced viability.A wound healing assay was performed to explore the effects of GSE, showing that it effectively inhibited AGS cell migration, indicating anti-metastatic properties.In addition, real-time PCR analysis identified the downregulation of several key oncogenes, including KRAS, BCL-2, JAK2, and C-Myc, in GSE-treated cells compared to the untreated controls.These oncogenes play crucial roles in tumor growth, cell survival, and metastasis.These findings suggest that GSE has anti-cancer effects by suppressing cell proliferation and migration in gastric cancer cells.These results highlight the potential of GSE as a promising natural therapeutic agent, warranting further investigation to elucidate its mechanisms and therapeutic applicability in vivo.
Authors: Junghyun KIM, Da-Hyun KIM
Institution: Kyungbok University
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4405911901