Work

Genome-wide identification and expression patterns of uridine diphosphate (UDP)-glycosyltransferase genes in the brown planthopper, Nilaparvata lugens

Year: 2024

Type: article

Abstract: Uridine diphosphate-glycosyltransferases (UGTs) are responsible for glycosylation by combining various small lipophilic molecules with sugars to produce water-soluble glycosides, which are crucial for the metabolism of plant secondary metabolites and detoxification in insects. This study presents a genome-wide analysis of the UGT gene family in the brown planthopper, Nilaparvata lugens , a destructive insect pest of rice in Asia. Based on the similarity to UGT homologs from other organisms, 20 putative NlUGT genes were identified in N. lugens . Sequence analysis revealed an average amino acid identity of 45.64 %; however, catalytic and sugar-binding residues, along with UGT signature motifs, were highly conserved. Phylogenetic analysis showed that the 20 NlUGTs were clustered into three main groups. The motif numbers ranged from 5 to 10, with motifs 1 and 4 being found in the functional domains of all 20 NlUGT proteins. Tandem and segmental duplication analysis identified one tandem duplication pair ( UGT386K7 and UGT386K8 ) and two pairs of collinearity genes ( UGT362C6 / UGT386J4 and UGT386C2 / UGT386G5 ) that expanded through segmental duplication within the UGT gene family of N. lugens . Combining the transcriptome and real-time quantitative PCR data showed that gut, antennae, integument, and ovaries were the tissues enriched with NlUGT gene expression. Six NlUGTs were present mainly in the gut, suggesting their putative roles in detoxification. This research provides valuable information on the molecular and genetic basis of NlUGTs , establishing a solid foundation for subsequent functional investigations of UGT s in planthopper, as well as paving the way for identifying potential targets to manage N. lugens effectively. A total of 20 UDP-glycosyltransferase (UGT) genes were identified in Nilaparvata lugens , which were classified into four subfamilies based on phylogenetic analysis. We found tandem and segmental duplication events drive expansion of the UGT gene family in Nilaparvata lugens . Some NlUGTs were presented mainly in the gut, indicating their potential involvement in detoxification processes. • The 20 NlUGT genes in Nilaparvata lugens were identified. • The sequence and phylogenetic of NlUGT genes were analyzed. • Tandem and segmental duplication events drive expansion of the UGT gene family in N. lugens . • The expression of NlUGT genes in different development stages and tissues were analyzed. • Some NlUGTs were presented mainly in the gut.

Source: Comparative Biochemistry and Physiology Part D Genomics and Proteomics

DOI: 10.1016/j.cbd.2024.101403