Work

Double-Stranded RNA-Based Method for Diagnosing Severe Fever with Thrombocytopenia

Year: 2024

Type: article

Abstract: Background/Objectives: This study explores the potential of using elevated levels of blood double-stranded RNA (dsRNA) as a diagnostic tool for severe fever with thrombocytopenia syndrome (SFTS) infection. Methods: Blood samples from SFTS patients were collected, dsRNA was purified, and total dsRNA expression was quantitatively analyzed using a spiropyran-based method. Comparative analysis was performed using blood samples from healthy individuals and scrub typhus patients with similar symptoms. Results: The results revealed that individuals infected with SFTS had significantly higher total blood dsRNA levels compared to healthy or scrub typhus controls. The dsRNA-based method also has potential for assessing infection severity based on dsRNA levels. Conclusions: These findings suggest that total dsRNA expression can serve as a quick and convenient method to differentiate SFTS from other non-viral conditions with similar clinical presentations. This method shows promise as a novel diagnostic tool.

Source: Journal of Clinical Medicine

DOI: 10.3390/jcm14010105

Authors: Jung Wan Park, Jaemin Jeon, Yoosik Kim, Min Hyok Jeon

Institutions: Soonchunhyang University Hospital Cheonan, Korea Advanced Institute of Science and Technology

Language: English

Topic: Viral Infections and Vectors

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,955