The Use of a Novel Container for Secured Transport and Storage of Biological Material for Quantitative Human RNA Analysis
Year: 2024
Type: article
Abstract: The transport of biological materials must protect samples from degradation and ensure courier safety. The main goal of this study was to evaluate the usefulness of a new type of container designed for the secured transport of biological material for storing samples for quantitative RNA analyses. This was achieved by analyzing changes in the expression of selected human leucocyte housekeeping genes (ACTB, GAPDH, and Rack1) using reverse transcription quantitative PCR (RT-qPCR) and digital PCR (RT-dPCR) techniques. Digital PCR analysis evidenced that the novel type of container retains a higher count of analyzed gene copies per µL of samples during 5 h of incubation time. The container ensures a low maintenance temperature for several hours, making it useful for sustaining the conditions for transporting biological samples. This novel container can be used to store and transport biological material to be analyzed by molecular techniques and can retain the stability of total RNA over several hours.
DOI: 10.3390/ijms26010228
Institutions: Pomeranian Medical University, University of Szczecin
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,296
OpenAlex ID: W4405920831