Strain-limited biofilm regulation through the Brg1-Rme1 circuit in Candida albicans
Year: 2024
Type: article
Abstract: ABSTRACT Prominent virulence traits of Candida albicans include its ability to produce filamentous hyphal cells and grow as a biofilm. These traits are under control of numerous transcription factors (TFs), including Brg1 and Rme1. In the reference strain SC5314, a brg1 Δ/Δ mutant has reduced levels of biofilm/filament production; a brg1 Δ/Δ rme1 Δ/Δ double mutant has wild-type levels of biofilm/filament production. Here, we asked whether this suppression relationship is preserved in four additional strain backgrounds: P76067, P57055, P87, and P75010. These strains represent diverse clades and biofilm/filament production abilities. We find that a rme1 Δ/Δ mutation restores biofilm/filament production in a brg1 Δ/Δ mutant of P76067, but not in brg1 Δ/Δ mutants of P57055, P87, and P75010. We speculate that variation in activities of two functionally related TFs, Nrg1, and Ume6, may cause the strain-limited impact of the rme1 Δ/Δ mutation. IMPORTANCE Candida albicans is a widespread fungal pathogen. The regulatory circuitry underlying virulence traits is well studied in the reference strain background, but not in other clinical isolate backgrounds. Here, we describe a pronounced example of strain variation in the control of two prominent virulence traits, biofilm formation and filamentation.
Source: mSphere
Authors: Min-Ju Kim, Aaron P. Mitchell
Institution: University of Georgia
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,500
OpenAlex ID: W4405920966