Year: 2024
Type: article
Abstract: RATIONALE: The performance of the capillary column directly impacts the separation efficiency of complex sample in liquid chromatography-mass spectrometry-based proteomics studies. The hydraulic packing system offers an effective solution by reducing packing time and expediting the preparation process of column preparation. However, its operational complexity and strict parameter regulation requirements hinder efficient application. METHODS: This study developed an innovative device for the hydraulic capillary column packing, followed by a systematic optimization and evaluation of crucial parameters such as packing pressure, magnetic stirring rate, concentration of solid-phase material, and choice of packing solvent in the capillary column packing process. RESULTS: The comprehensive evaluation of the prepared capillary columns showed that the hydraulic packing method enabled rapid and large-scale preparation, while also demonstrating exceptional stability and reproducibility. CONCLUSIONS: The proposed hydraulic capillary column packing strategy in this study holds theoretical and practical implications for advancing the technology of column preparation, enhancing separation efficiency, and expanding the depth of analysis in proteomics research.
DOI: 10.1002/rcm.9966
Authors: Chunqiu Hu, Hang Fu, Lulu Wang, Yuan Gao, Ruidong Li, Zhen Li, Shuai Li, Xiaowang Xie, Chongxi Ren, Fang Guo, Minjia Tan, Linhui Zhai
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
Awards: G2642236564, G8464521582
OpenAlex ID: W4405923086