Evaluation of Antioxidant and Antibacterial Activities of Leaf and Seeds Extracts of Neem (Azadirachta indica) Using Different Extraction
Year: 2024
Type: article
Abstract: Neem (Azadirachta indica), has long been recognized for its medicinal properties. This study evaluates the antibacterial, antioxidant using the Ferric-Biperidine Reducing Capacity of Total Antioxidants (FBRC), Ferric-Reduced Antioxidant Method (FRAP), and scavenging activity by the 1, 1-diphenyl 2-picrylhyorazyl (DPPH) method. The total phenolic content of neem leaf and seed extracts was determined using Folin-Ciocalteu Aliquot method. Among the extracts analyzed, the aqueous soaked leaf extract exhibited the highest antioxidant activity, followed by the ethanolic-soaked leaf extracts, while the ethanolic Soxhlet seed extracts demonstrated the lowest activity. the total phenolic content was the highest in ethanolic Soxhlet seed extracts (6.319 mg/mg AAE) and aqueous soaked leaf extracts (4.838 mg/mg), with ethanolic soaked leaf extracts showing the lowest concentration (4.165 mg/mg). Antibacterial activity against Escherichia coli and Staphylococcus aureus was observed exclusively in ethanol-soaked leaf extracts, with significant inhibition of S. aureus (18 mm). These findings high-light the potent antioxidant and antimicrobial properties of neem extracts, supporting their potential application in medicinal treatments.
Institutions: Sana'a University, Amran University
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4405926345