Work

IS15DIV-flanked composite transposon harboring blaNDM-5 in multidrug-resistant Salmonella Typhimurium

Year: 2024

Type: article

Abstract: Summary Multidrug-resistant Salmonella Typhimurium has emerged as a global public health concern. Asymptomatic gastrointestinal carriage is a key factor in the spread of antibiotic-resistant bacteria. However, it is challenging to obtain direct evidence of in vivo transfer of mobile genetic elements (MGEs). Here, we found that MGEs harboring blaNDM-5 were transferred from asymptomatic Escherichia coli (LS20223694) to Salmonella Typhimurium (LS20223695) in a child. BLAST analyses demonstrated that the IS15DIV-flanked composite transposon of pLS20223695_NDM5 showed high similarity with pLS20223694_NDM5 and two previously reported plasmids, suggesting the possibility of genetic recombination. Besides, conjugation experiments showed that the transconjugant carried the incompatibility group I1 (IncI1)-I(alpha) plasmid replicon with the blaNDM-5 and sul1 genes, indicating that pLS20223695_NDM5 is a conjugative plasmid with transferability. Our study provides insights into the genetic basis of an IS15DIV-flanked composite transposon in Salmonella Typhimurium.

Source: iScience

DOI: 10.1016/j.isci.2024.111720

Authors: Kaiting Zhao, Jing Jin, Yuan Liao, Aixia Liu, Wugao Liu, Weiping Wu

Institutions: Wenzhou Medical University, Lishui City People's Hospital

Language: English

Topic: Antibiotic Resistance in Bacteria

Subfield: Molecular Medicine

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,150