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Mettl14 and Mettl3 Work Cooperatively to Regulate Retinal Development

Year: 2024

Type: article

Abstract: ABSTRACT N6‐methylenadenosine (m6A) modification, the most abundant epitranscriptomic modification in eukaryotic mRNAs, has been shown to play crucial roles in regulating various aspects of mRNA metabolism and functions. In this study, we applied the Cre‐Loxp conditional knockout system to investigate the role of the core components of the m6A methyltransferase complex, METTL14 and METTL3, in retinal development. Our results showed that the double absence of Mettl14 and Mettl3 caused structural disturbance in the retina and prolonged the proliferation activity of retinal progenitor cells. Interestingly, the deletion of Mettl14 and Mettl3 did not affect the generation of various retinal cells, but severely disrupted their distribution. In addition, double deletion of Mettl14 together with Mettl3 caused a stronger phenotype than did single deletion of Mettl14. In conclusion, our study demonstrated that Mettl14 and Mettl3 work cooperatively to regulate retinal development.

Source: Cell Biochemistry and Function

DOI: 10.1002/cbf.70039

Authors: Dan Chen, Yanling Xin, Jingyi Guo, Shuyi Chen

Institutions: Sun Yat-sen University, State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University

Language: English

Topic: Retinal Development and Disorders

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Chinese Academy of Sciences, Guangdong Science and Technology Department, State Key Laboratory of Ophthalmology

OpenAlex ID: W4405948798

FWCI: 0.116

Cites: 35

Cited by: 1

Related to: 10