Work

A Mitochondria‐Targeted Iridium(iii) Phosphorescent Probe for Selective Detection of Hypochlorite in Living Cells

Year: 2024

Type: article

Abstract: Hypochlorous acid(HClO)/hypochlorite ion (ClO−) is a highly reactive oxygen species (ROS) that play a crucial role in various biological processes. In this paper, a “turn‐on” phosphorescent probe (Ir‐TPP) for detecting ClO− in mitochondria was designed and synthesized. In solution, Ir‐TPP is minimal emission due to rapid isomerization of C=N−OH as an efficient non‐radiative decay process. When Ir‐TPP was reacted with ClO− to form an oxidized carboxylate product, there is a significant enhancement both in absorbance and emission intensity, resulting in approximately 25‐fold increase in phosphorescence signal. Furthermore, the probe demonstrates high selectivity towards other anions and ROS. It also exhibits specific targeting ability towards mitochondria with a co‐staining coefficient of 94 % compared to commercial dyes, enabling “turn‐on” detection of ClO− within mitochondria.

Source: Chemistry - An Asian Journal

DOI: 10.1002/asia.202401351