Work

The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes

Year: 2024

Type: article

Abstract: This works focuses on developing an optimized methodology for analyzing free and bound volatile compounds in grapes using enzymatic hydrolysis with AR2000 enzyme and HS-SPME-GC × GC-ToFMS. To achieve this purpose, a previous optimization step of enzymatic hydrolysis was implemented and optimal conditions for enzymatic hydrolysis were established using 50 mg of AR2000 enzyme at 35 °C for 24 h, while the best extraction conditions were found to be 40 min at 60 °C. Applying this methodology to clones of Moreto grapes from harvest of 2022 and 2023, it was possible to identify and semi-quantify sixty volatile compounds, with more compounds identifiable in 2022 than in 2023. The results also showed the influence of crop year and genetic intravariability. • Analysis of free and bound volatile compounds in one injection. • Enzymatic hydrolysis coupled to HS-SPME - GC x GC-ToFMS. • 60 varietal volatile compounds were identified in Moreto grapes. • Harvest year and genetic variability influence varietal volatile profile of grapes.

Source: LWT

DOI: 10.1016/j.lwt.2024.117314

Authors: Daniela Fonseca, Nuno Martins, Raquel Garcia, Maria João Cabrita

Institution: University of Évora

Language: English

Topic: Horticultural and Viticultural Research

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $2,570