Work

Fabrication of immobilized lipases for medium and long‐chain triacylglycerols preparation through solvent‐free acidolysis

Year: 2024

Type: article

Abstract: The present study aims to fabricate efficient immobilized lipases for medium and long‐chain triacylglycerols preparation through acidolysis. Eighteen novel materials, including five mesoporous materials, four organic groups modified SBA‐15 samples (R‐SBA‐15) and nine macroporous resins, were employed as matrices for lipases immobilization. Lipases of TLL, AOL, RML and CALB were evaluated. Results indicated that TLL exhibited better acidolysis performance and decanoic acid content from 30% to 45% could be obtained; while other lipases (CALB, RML, and AOL) showed poor performance. In addition, macroporous resins of DA‐201, ADS‐17, D3520, and mesoporous silicas of SBA‐15, Al‐MCM‐41, HMS, were suitable for TLL immobilization. TLL@SBA‐15 was selected for acidolysis and the reaction conditions were optimized through single factors study and orthogonal design. The optimum conditions for the acidolysis were: lipase amount 6 wt%, decanoic acid: camellia oil molar ratio 14:1, reaction time 12 h and temperature 50°C. Under these conditions, decanoic acid content at 59.87%, oleic acid content 33.99% and sn‐2 decanoic acid content 7.41% was obtained (the mentioned fatty acids here were the fatty acids combined to oil molecules, not free fatty acids).

Source: Journal of the American Oil Chemists Society

DOI: 10.1002/aocs.12926

Authors: Shuping Lin, Can Zeng, Qiangyue Zhang, Nanjing Zhong

Institution: Guangdong Pharmaceutical University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: National Natural Science Foundation of China

Award: G2146690437