Work

Enzymatic Hydrolysis Systems Enhance the Efficiency and Biological Properties of Hydrolysates from Frozen Fish Processing Co-Products

Year: 2024

Type: article

Abstract: Co-products from the frozen fish processing industry often lead to financial losses. Therefore, it is essential to transform these co-products into profitable goods. This study explores the production of fish protein hydrolysates (FPH) from three co-products: heads and bones of black scabbardfish (Aphanopus carbo), carcasses of gilthead seabream (Sparus aurata), and trimmings of Nile perch (Lates niloticus). Four enzymatic hydrolysis systems were tested: an endopeptidase (Alcalase, A), an exopeptidase (Protana, P), two-stage hydrolysis with an endopeptidase followed by an exopeptidase (A+P), and a single stage with endo- and exopeptidase (AP). The results show that combined enzymatic treatments, especially single stage Alcalase and Protana (AP), achieved high protein yields (80%) and enhanced degrees of hydrolysis (41 to 61%), producing peptides with lower molecular weights. FPH exhibited significant antioxidant activity, with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays, with EC50 values below 5 mg/mL. Additionally, AP hydrolysates demonstrated over 60% ACE (angiotensin converting enzyme) inhibition at 5 mg/mL, indicating potential antihypertensive applications. The antidiabetic and anti-Alzheimer activities were present but at relatively low levels. AP hydrolysates, especially from gilthead seabream, proved to be the most promising. This study highlights the value of fish co-products as sources of functional peptides, contributing to waste reduction and potential applications in food, agriculture, and as nutraceuticals.

Source: Marine Drugs

DOI: 10.3390/md23010014

Authors: Maria Sapatinha, Carolina Camacho, Juliana Costa, Ana Luísa Fernando, António Marques, Carla Pires

Institutions: Portuguese Sea and Atmosphere Institute, Universidade Nova de Lisboa, Universidade do Porto, Centro Interdisciplinar de Investigação Marinha e Ambiental

Language: English

Topic: Protein Hydrolysis and Bioactive Peptides

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: gold

Estimated APC paid: $3,296