Year: 2024
Type: article
Abstract: Objective: Exploration of molecular markers is an ongoing focus in the field of bladder cancer research.Based on data from public databases, GATA3-AS1 was identified as upregulated in bladder urothelial carcinoma (BLCA); however, its exact function and regulatory mechanism in this context remain unclear.Methods: To investigate the clinical implications of GATA3-AS1, we examined its levels in 90 BLCA and adjoining normal tissue samples.Functional assays were conducted to assess the effects of GATA3-AS1 on BLCA cell proliferation, migration, and invasion.Animal assays were employed to determine the effects of GATA3-AS1 on BLCA tumorigenicity in vivo.Immunoblotting, RNA pull-down, RNA immunoprecipitation, TOP/FOP luciferase reporter gene, and coimmunoprecipitation assays were used to explore the molecular mechanism underlying the effects of GATA3-AS1 on BLCA progression.Results: GATA3-AS1 expression was significantly up-regulated in BLCA tissues and correlated with pathological stage, grade, and poor patient outcome.Altered GATA3-AS1 levels promoted BLCA proliferation, migration, and invasion.Mechanistic studies suggested that GATA3-AS1 interacts with DDX5 protein, enhances its stability, and ultimately leads to BLCA progression through Wnt/β-catenin signaling pathway activation.Conclusion: GATA3-AS1 overexpression increases the aggressiveness of BLCA by activating the Wnt/β-catenin pathway through binding to DDX5.GATA3-AS1 has potential as a new molecular predictor of poor prognosis in patients with BLCA.
Source: Journal of Cancer
DOI: 10.7150/jca.104034
Authors: Yatao Duan, Zonghang Liu, Yongliang Ma, Pan Qi, Yanli Guo, Aili Zhang
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,337
OpenAlex ID: W4405954224