Work

Unveiling the benefit of germinated colored rice extract to decrease metastatic potential of colorectal cancer cells

Year: 2024

Type: article

Abstract: There is increasing evidence of the health effects of germinated colored rice (GCR). Metastasis, namely the spread of cancer cells to distant sites in the body, is a serious problem for cancer therapy. This study aimed to explore the anti-metastatic potential of GCR extract in HT29 colorectal cancer cells through cell migration inhibition, and to identify target proteins by using proteomic approach. GCR extract at 4 mg/mL caused 87 % reduction in cell migration. Two-dimensional gel proteomic analysis revealed β-actin downregulation in GCR-treated cells. GCR also affected gene expression of actin dynamic regulatory proteins including RHOA , RAC1 , and CDC42 genes. Gamma-aminobutyric acid (GABA), a bioactive compound present in GCR extract, could decrease cell migration and downregulated β-actin in the cells, but did not alter expression level of RHOA , RAC1 , and CDC42 genes. This study reveals a potential of GCR to be developed as a functional food for assisting the cancer treatment. • Germinated colored rice (GCR) has a beneficial effect to prevent cancer progression. • GCR extract decreases cell migration capability of human colorectal cancer cells. • GCR extract interferes expression of cell migration-associated proteins. • GABA is the active compound contributes to the beneficial effect of GCR extract.

Source: Journal of Functional Foods

DOI: 10.1016/j.jff.2024.106655