Targeted detection of sequence variants in cell-free DNA from cerebrospinal fluid in pediatric central nervous system tumors
Year: 2025
Type: article
Abstract: The emergence of liquid biopsy technologies holds great promise in the cancer setting, including in pediatric central nervous system (CNS) tumors. In contrast to broad lower-depth sequencing, commonly referred to as low pass whole genome sequencing (WGS), targeted platforms with a higher depth of coverage have also been established. Here, we review targeted liquid biopsy techniques with applicability to pediatric CNS tumors. These include polymerase chain reaction (PCR), both droplet digital PCR and reverse transcription-based PCR, Sanger sequencing, and next-generation sequencing approaches that incorporate amplicon- and hybrid capture-based methods. The goal of this paper is to facilitate an understanding of these targeted techniques and provide a context for clinical relevance within disease categories, as well as a discussion on optimizing real-world implementation for pediatric CNS tumors.
Source: Frontiers in Oncology
Institutions: University of Southern California, Children's Hospital Los Angeles, Seattle Children's Hospital, University of Washington, Fred Hutch Cancer Center, Brigham and Women's Hospital, Harvard University, NYU Langone Health, NYU Langone’s Laura and Isaac Perlmutter Cancer Center, New York University
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4406082925