Work

First VIM-producing representative of Pseudomonas putida group from the largest Bulgarian hospital

Year: 2024

Type: article

Abstract: Here we describe the first detected VIM-2-producing representative of Pseudomonas putida group – Pseudomonas kurunegalensis from the largest Bulgarian hospital – St George University Hospital in Plovdiv. A 59-year-old female patient with right-sided lung abscess was hospitalized in the Second Clinic of Thoracoabdominal Surgery. She was repeatedly treated for pulmonary infections. Punctate from the abscess cavity was taken for microbiological investigation. Identification process and antimicrobial susceptibility were performed by Vitek 2. The species group P. putida was confirmed with MALDI-TOF system and whole genome sequencing defined it as P. kurunegalensis. Antibiotic susceptibility testing revealed susceptibility only to tobramycin and colistin. All phenotypic tests for carbapenemase and metallo-beta-lactamase (MBL) production were positive. Multiplex PCR was performed to search for nine common carbapenemase encoding genes whereas the variable region of the integron was determined by DNA sequencing. Molecular assays confirmed the presence of blaVIM-2 located within a typical Class I integron including also an aacA29b aminoglycoside N(6’)-acetyltransferase casette. Despite P. putida not being a common pathogen, it still could survive in hospital conditions causing difficult-to-treat infections and becoming a source of resistant genes, including MBL-encoding genes.

Source: Folia Medica

DOI: 10.3897/folmed.66.e129478