Year: 2024
Type: article
Abstract: The objective of our research was to conduct a comparative analysis of cryopreserved sperm quality parameters of Tsigai (TS) sheep breed. Ejaculates (n=12) from TS sheep rams (n = 2) were collected using electro-ejaculation. Samples were prepared from suitable ejaculates, ensuring at least 70% progressive motility. These samples were then equilibrated in Triladyl® diluent and subjected to automated freezing. Before freezing and after thawing, the sperm samples underwent assessments including motility (CASA), viability and apoptosis (DRAQ7/Yo-Pro-1), mitochondrial activity (MitoTracker), capacitation status (FLUO4), and acrosomal status (PNA). The results revealed significant differences (P < 0.05) in total motility between fresh (91.25±0.80 %) and frozen/thawed (F/T) (41.14±12.04 %) samples, as well as progressive motility between fresh (89.83±1.85 %) and frozen/thawed (33.57±8.25 %) groups. Moreover, significant differences (P<0.05) were also observed in the proportion of apoptotic spermatozoa (2.26±0.60 %), dead (7.40±1.72 %), and mitochondrial active spermatozoa (84.52±5.60 %) in fresh samples compared to F/T samples (12.34±0.80 %, 46.59±2.82 %, 47.71±4.04 %). Our findings indicate that the selected cryopreservation protocol is relatively sufficient for the cryoconservation of sperm from Tsigai sheep breeds, since up to 50% of F/T sperm were motile and live. This has significant implications for biodiversity protection and simplifies the establishment of an animal genetic resources gene bank.
Author: Peter Chrenek
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
SDG: Zero hunger
Open Access status: diamond
Awards: G1954507429, Ochrana ohrozených slovenských plemien hospodárskych zvierat v podmienkach ex situ, Identifikácia a kryouchovávanie spermatogoniálnych kmeňových buniek slovenských plemien králikov, baranov a býkov, G2950615972, Kryokonzervácia živočíšnych genetických zdrojov slovenských plemien, G4221948015, Ochrana vybraných slovenských plemien v podmienkach in vitro, G6227076073, G6535325354, G7761450427, G7945670258
OpenAlex ID: W4406109858