Year: 2025
Type: preprint
Abstract: This protocol describes the use of Deterministic Barcoding in Tissue for spatial omics sequencing (DBiT-seq) platform to construct a multi-omics atlas on FFPE tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section on a glass slide. This technique does not directly resolve single cells but can achieve a near-single-cell resolution for spatial transcriptomics. This protocol is adapted from the Patho-DBiT publication - Spatially exploring RNA biology in archival formalin fixed paraffin-embedded tissues (Bai et al., 2024, Cell 187, 6760–6779)
Authors: Archibald Enninful, Rong Fan, Negin Farzad
Institution: Yale University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4406137665