Work

Plasticity of BioPhi-driven humanness optimization in ScFv-CD99 binding affinity validated through AlphaFold, HADDOCK, and MD simulations

Year: 2025

Type: article

Abstract: displaying improved humanness, a larger complementarity-determining region (CDR) cavity, and a more hydrophobic CDR-H3 loop. These findings highlight the delicate balance between enhancing humanness and preserving the structural and functional integrity critical for therapeutic antibody development.

Source: Computational and Structural Biotechnology Journal

DOI: 10.1016/j.csbj.2025.01.001

Authors: Kanokporn Sornsuwan, Thanathat Pamonsupornwichit, On-anong Juntit, Weeraya Thongkum, Nuchjira Takheaw, Kanchanok Kodchakorn, Chatchai Tayapiwatana

Institutions: Chiang Mai University, National Science and Technology Development Agency, National Center for Genetic Engineering and Biotechnology

Language: English

Topic: Monoclonal and Polyclonal Antibodies Research

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,230

Funders: Chiang Mai University, Thailand Science Research and Innovation

Awards: G3336452283, G5883953123, G7679188404, G8555061488

OpenAlex ID: W4406140317

FWCI: 2.639

Cites: 67

Cited by: 5

Related to: 10