Production, Large-Scale Extraction, and Purification of Phycocyanin by Different Cyanobacteria Isolated from Various Environments
Year: 2024
Type: article
Abstract: The current research aims to investigate the isolates with the highest phycocyanin yield among five cyanobacterial isolates obtained from the wastewaters of the Porsuk River, thermal springs, and boron-containing waters of Eskişehir province in Türkiye. The study focused on optimization, purification, and characterization to increase phycocyanin yield. Phycocyanin yield was initially analyzed using four extraction methods: freeze-thaw, sonication, homogenization, and treatment with lysozyme. The sonication method determined the thermophilic Geitlerinema sp. as the isolate with the highest phycocyanin yield (116±0.18 mg g-1 C-phycocyanin). The crude extract obtained following optimization studies was first purified by solid ammonium sulfate precipitation/dialysis at 50% and 75% saturation, followed by ion-exchange chromatography using two anionic resins (DEAE-cellulose and Q-sepharose). The purity rate determined in the spectrum was recorded as 4.12 with 48.9% recovery (A620/A280). Characterization using the SDS-PAGE method found molecular weights of 17 kDa and 19 kDa for subunit α and subunit β, respectively. Additionally, we evaluated the stability of the purified thermal phycocyanin at different temperatures, with the pigment displaying resistance to high temperatures up to 60°C, as shown by its CR value.
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: diamond
Funder: Eskişehir Teknik Üniversitesi
OpenAlex ID: W4406143733