Figure 3 from Claudin-4 Stabilizes the Genome via Nuclear and Cell-Cycle Remodeling to Support Ovarian Cancer Cell Survival
Year: 2025
Type: other
Abstract: Remodeling of nuclear morphology and the nuclear lamina during claudin-4 disruption. Ovarian tumor cells were treated with CMP (400 µmol/L) for 48 hours, and then cells were stained to mark the nuclear lamina (using antibodies against lamin B1 and lamin A/C) and the nuclei (using DAPI). Subsequently, we performed a morphometric characterization. A, Top, confocal images showing (maximum projections) nuclei size of OVCAR8 as well as OVCA429 (B) and OVCAR3 cells (C). Bottom, corresponding quantification for (A–C; n = OVCAR8, 1711 cells; OVCA429, 2,630 cells; OVCAR3, 2,365 cells; two-tailed Mann–Whitney test, Kruskal–Wallis test with Dunn’s multiple comparisons; three independent experiments). D, Top, selected confocal images (maximum projections) showing nuclear lamina components corresponding to OVCAR8 cells overexpressing claudin-4 and claudin-4 downregulation in OVCA429 (E) and OVCAR3 cells (F). Bottom, corresponding quantification of nuclear accumulation of lamin B1 and lamin A/C (yellow arrowheads highlight comparison of lamin B1 during claudin-4 overexpression and downregulation) under different conditions (three independent experiments; Kruskal–Wallis test with Dunn’s multiple comparisons. (Significance, P < 0.05). Graphs show mean and SEM, scale bar, 5 µm. KD, knockdown; MFI, mean fluorescence intensity; Veh, vehicle.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4406148053