Work

Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging

Year: 2025

Type: article

Abstract: Combining transparent embedding with sectioning is likely to be the future direction for tissue clearing and 3-dimensional (3D) imaging. A newly published transparent embedding system, TESOS (Transparent Embedding Solvent System), ensures consistent submicron resolution imaging throughout the entire sample, and can be compatible with different microscopy systems. This method shows great potential in connectome mapping, and might be an optimal option for future 3D multiplex immunofluorescence and RNA in situ hybridization imaging. Additional efforts would be needed to innovate labeling, imaging, and data processing strategies to fully utilize the potential of transparent embedding systems in high-resolution imaging of large-scale samples.

Source: BME Frontiers

DOI: 10.34133/bmef.0095

Authors: Yating Yi, Hu Zhao

Institutions: Sichuan University, State Key Laboratory of Oral Diseases, Chinese Institute for Brain Research

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Natural Science Foundation of China, Sichuan University, West China School of Stomatology, Sichuan University

Awards: G312501742, G8095708686