Work

Protective Effects of Hydrogen Treatment Against High Glucose-Induced Oxidative Stress and Apoptosis via Inhibition of the AGEs/RAGE/NF-κB Signaling Pathway in Skin Cells

Year: 2025

Type: article

Abstract: BACKGROUND: Diabetic wounds are major clinical challenges, often complicated by oxidative stress and free radical generation. Hydrogen (H2), a selective antioxidant, offers potential as a therapeutic agent for chronic diabetic wounds. However, its precise mechanisms remain underexplored. OBJECTIVE: on high glucose-induced oxidative damage and apoptosis in human skin cells. METHODS: HaCaT keratinocytes and HSF fibroblasts were treated with high glucose or AGEs. Cell viability, oxidative stress markers, inflammatory cytokines, and apoptosis were analyzed. AGEs/RAGE/NF-κB signaling was evaluated via Western blot. RESULTS: treatment significantly reduced ROS, MDA, IL-1β, and TNF-α levels, while enhancing SOD and GSH activity. It also inhibited AGEs/RAGE/NF-κB signaling and apoptosis. CONCLUSION: Hydrogen therapy protects against oxidative stress and inflammation induced by high glucose or AGEs, offering potential as an adjunctive treatment for diabetic wound healing.

Source: Endocrine Metabolic & Immune Disorders - Drug Targets

DOI: 10.2174/0118715303369584241231141001

Authors: Pan Yu, Nan Hong, Qiong Wu, Zhipeng Zhao

Institutions: Nanjing General Hospital of Nanjing Military Command, Nanjing University

Language: English

Topic: Hydrogen's biological and therapeutic effects

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Jiangsu Commission of Health