Extraction And Evaluation Of Antidotes From Musca Domestica (Houseflies) Wings Against Its Body Pathogens
Year: 2025
Type: article
Abstract: Musca domestica is an important insect vector of various myriads of microbes ranging from bacteria, fungi and parasites. They tend to infest food and drinks of human and animals causing severe diseases. Unfortunately, some of the microbes/diseases transmitted by this vector have developed resistance against various drugs (Drug Resistance Microbes). This current study evaluates the microbial loads on various body parts (Right wing, Left wing and Body Surface) of M. domestica captured from different locations in Ilaro, Ogun State and the antimicrobial properties of the body parts. One hundred and twenty samples of M. domestica were trapped from different locations including abattoirs, food vendors, house hold waste bin area and palm wine shops. Trapped flies were examined for Total Bacteria Count (TBC), Total Coliform Count (TCC) and Total Fungi Count (TFC) and the presence of antimicrobial agent on their body parts. Highest TBC and TCC (2.42x102 and 1.47x102 ) were found in flies trapped in Palm Wine Body Parts (PBP), while highest TFC (2.40x102 ) was found in flies captured in Abattoir Body Parts (ABP). No bacteria, coliform and fungi growth were observed in the right wing of flies trapped in the four locations except the Food-Vendor Right Wings-0.18x102 . (FRW). Cultural and biochemical characteristics of the bacteria isolate shows the presence of Proteus spp, Salmonella spp, Escherichia coli, Staphylococus aureus, Bacillus spp and Klebsiella spp. No bacteria growth was found in Palm-wine Right wings (PRW). No fungi growth was found in the PRW, House-hold Right wings and FRW. Other body parts carry various fungi including Mucor spp, Aspergillus spp, Fusarium spp, Rhizopus spp and Penicillium spp. Statistically, antimicrobial test of the water and ethanolic extract of the right-wing against test organism shows no significant difference (p>0.05) when compared. Dimethyl Sulphoxide (DMSO) shows no significant effect (p>0.05) against all the test organisms. Significantly (p<0.05), Ciprofloxacin shows better effect than the water and ethanolic extracts of the right wings as it exhibited the highest zone of inhibition (30.33± 0.58) and sensitivity against all the organisms. The right-wing extracts can be examined for other antimicrobials properties and can be enhanced to develop natural and novel antimicrobial agent for controlling microbes in food and water.
Institution: The Federal Polytechnic Ilaro
Language: English
Subfield: Health, Toxicology and Mutagenesis
Field: Environmental Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W4406253203