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Characteristic of gut microbiota in young people in Arkhangelsk

Year: 2024
Type: article
Study design: Observational Study
Abstract: The study of the biodiversity of the colon microbiota in residents of the Arctic regions is currently of particular interest. The combination of cultural and molecular genetic methods allows for a comprehensive assessment of the microbiota spectrum. Purpose of the study – to assess the colon microbiota in young, healthy residents of Arkhangelsk. The material for the study was feces from healthy residents (n = 43). Analysis of the nature of the microbiota was carried out using cultural methods and PCR with the determination of 33 indicators. Research results. According to PCR data, 100 % of the subjects had a deficiency of lactobacilli; Roseburia inulinivorans was present in 20.9 %, Blautia spp. in 72.1 %, Agathobacter rectalis in 37.2 %, E. coli in 60.46 %, and there was an excess of Fusobacterium nucleatum in 16.28% of cases, Streptococcus spp. in 13.95 %, Acinetobacter spp. in 6.97 %, and opportunistic representatives of the order Enterobacteriales in 32.58 %. According to the results of the cultural research method, a deficiency of lactobacilli was recorded in 97.67 % of cases, enterococci in 51.16 %, Bacteroides in 27.91 %, typical (lactose +) E. coli in 72.09 %, bifidobacteria in 13.95 %, as well as detection of an excess of Klebsiella spp. in 32.58 % and Staphylococcus aureus in 4.65%. The average amount of lactobacilli by the cultural method was 4.81 lg CFU/g, Enterococci 5.0 lg CFU/g, S. aureus 5.12 lg CFU/g, K. pneumoniae 4.75 lg CFU/g, K. oxytoca 5.62 lg CFU/g, E. coli 6.52 lg CFU/g, Bifidobacterium spp. 9.0 lg CFU/g, and Bacteroides spp. 9.0 lg CFU/g. Statistically significant differences in the identification of microorganisms were found for Lactobacillus spp., Enterococcus spp., Bifidobacterium spp., and Bacteroides spp. (p < 0.001). An imbalance of the colon microbiota in healthy residents of Arkhangelsk was revealed through cultural and molecular genetic research methods.
DOI: 10.17021/1992-6499-2024-4-46-59
Language: English
Open Access status: hybrid
OpenAlex ID: W4406268548
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Cites: 31
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Related to: 10