Alternative Indemnify More Effective Chemotherapy with Targeting Cancer Cell Using Encapsulation Technique
Year: 2024
Type: article
Abstract: Of all disorders, cancer ranks as one of the most fatal, causing up to 10 million fatalities in 2020. Chemotherapeutic drugs represent efficient therapy for a wide range of human cancers. The major obstacle in applying chemotherapy is the accompanying severe side effects. In the present study, nano-encapsulated bioactive compounds in the dietary supplements products with folate-targeting activity and pectin were designed to enhance the anticancer activity of two different formulas of nanoparticles: ND+F-dox (folic acid + doxorubicin in one capsule) and NDF-dox (folic acid, and doxorubicin in two separate capsules). Treatment of cancer cells with the two formulas significantly inhibited their proliferation in a concentration-dependent way with IC50 values of 4.44, 36.15 µg/ml in the case of HepG2 cells and 2.36, 22.58 µg/ml in MCF-7 cells, respectively. The formulas had high selectivity by reporting very low toxicity with MCF10A normal cells. The anticancer effect of current formulas was due to a significant elevation of DNA damage as NDF-dox and ND+F-dox significantly increased percentages to 20.49, and 46,48%, respectively. The formulas significantly induced apoptosis by upregulating apoptotic BAX and downregulating anti-apoptotic Bcl2 simultaneously with high selectivity and specificity. The present research offers compelling proof that ND+F-dox succeeded in boosting the anticancer efficacy of doxorubicin and a successful prospective strategy to lessen doxorubicin toxicity by targeting cancer cells, increasing its application for cancer treatment.
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: diamond
Funder: National Research Centre
Awards: G3297099542, G3950340701
OpenAlex ID: W4406272458