Year: 2025
Type: article
Abstract: Terminal olefins are important platform chemicals, drop-in compatible hydrocarbons and also play an important role as biocontrol agents of plant pathogens. Currently, 1-alkenes are derived from petroleum, although microbial biosynthetic routes are known. Jeotgalicoccus sp. ATCC 8456 produces 1-alkenes via the fatty acid decarboxylase OleTJE. UndA and UndB are recently identified non-heme iron oxidases converting medium-chain fatty acids into terminal alkenes. Our knowledge about the diversity and natural function of OleTJE, UndA, and UndB homologs is scarce. We applied a combined screening strategy-solid-phase microextraction coupled with gas chromatography-mass spectrometry (SPME GC-MS) and polymerase chain reaction (PCR)-based amplification-to survey an environmental strain collection for microbial 1-alkene producers and their corresponding enzymes. Our results reinforce the high level of conservation of UndA and UndB genes across the genus Pseudomonas. In vivo production of defined 1-alkenes (C9-C13; C15; C19) was directed by targeted feeding of fatty acids. Lauric acid feeding enabled 1-undecene production to a concentration of 3.05 mg l-1 in Jeotgalicoccus sp. ATCC 8456 and enhanced its production by 105% in Pseudomonas putida 1T1 (1.10 mg l-1). Besides, whole genome sequencing of Jeotgalicoccus sp. ATCC 8456 enabled reconstruction of the 1-alkene biosynthetic pathway. These results advance our understanding of microbial 1-alkene synthesis and the underlying genetic basis.
Source: FEMS Microbiology Letters
Authors: Matthias Schweitzer, Andrea Marianne Friedrich, Alexander Dennig, Gabriele Berg, Christina Andrea Müller Bogotá
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,297
Funders: TU Graz, Internationale Beziehungen und Mobilitätsprogramme, Amt der NÖ Landesregierung, Bundesministerium für Wissenschaft, Forschung und Wirtschaft, Österreichische Forschungsförderungsgesellschaft, Bundesministerium für Verkehr, Innovation und Technologie, Austrian Centre of Industrial Biotechnology, Steirische Wirtschaftsförderungsgesellschaft, Standortagentur Tirol, Technology Agency of the City of Vienna
OpenAlex ID: W4406317940