Yale University Co_Profile Spatial-ATAC andRNA Sequencing via Deterministic Barcoding in Fresh Frozen Human Lymph Node Tissue v1
Year: 2025
Type: preprint
Abstract: This protocol describes the use of Deterministic Barcoding in Tissue for spatial ATAC and RNA sequencing to construct epigenome and transcriptome atlases of fixed fresh frozen human lymph node tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tissue section fixed on a glass slide using DBiT-seq device. This method spatially resolved transcriptome and epigenome profiling of cellular senescence in fresh frozen human lymph node tissue samples.This technique does not directly resolve single cells but can achieve a near-single-cell resolution.
Institution: Yale University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4406375155