Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha Play a Role in PM2.5 and Hypoxia-Induced Simultaneously Oxidative Damage in Human Bronchial Epithelial Cells
Year: 2024
Type: article
Abstract: Peroxisome proliferator activated receptor γ co-activator 1α (PGC-1α) plays an important role in antioxidative stress.The aim of this study is to investigate the role of PGC-1α in the oxidative damage of BEAS-2B cells exposed to PM2.5 and hypoxia simultaneously and in the anti-oxidative effect of N-acetyl-L-cysteine (NAC).The PGC-1α mRNA level in human bronchial epithelial BEAS-2B cells exposed to PM2.5, hypoxia and PM2.5 combined with hypoxia were evaluated.The cells were treated for 12 h in the presence or absence of PM2.5 combined with hypoxia and in the presence or absence of NAC.The cell viability after intervention with NAC was assessed.PGC-1α was knocked down by siRNA to investigate the protective mechanism of NAC.The expression levels of PGC-1α and PPARγ were determined at both the mRNA and protein levels.The expression of NF-κBp65, malondialdehyde (MDA) and superoxide dismutase (SOD) were detected at the protein level.PM2.5 combined with hypoxia reduced the expression of PGC-1α and PPAR-γ (P<0.01),increased the protein levels of NF-κBp65 (P<0.01) and MDA(P<0.05), and decreased the SOD activity (P<0.05)compared to the normal control group, and NAC ameliorated the damage(P < 0.05).PGC-1α siRNA blocked the protective effect of NAC.PM2.5 combined with hypoxia can cause oxidative damage to BEAS-2B cells, and PGC-1α may play an important role in the anti-oxidative effect of NAC.The results of this study suggest for the first time that PGC-1α might play an important role in regulation of oxidative damage induced by PM2l.5 and hypoxia simultaneously in human bronchial epithelial cells, which provides potential ideas for the treatment of PM2.5-induced respiratory diseases.
Authors: Qian Tian, Xiaowei Gong, Zheng Duan, Yadong Yuan
Institution: Hebei Medical University
Language: English
Subfield: Health, Toxicology and Mutagenesis
Field: Environmental Science
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4406437862