Potential Anticancer Activity of Alcea rosea and Molecular Docking of Selected Target Proteins in Liver Cancer Cells
Year: 2024
Type: article
Abstract: The incidence of cancer is rising globally.In this study, the cytotoxic effects of Alcea rosea L., hexane seed (ARHS) extract were examined against liver (HuH-7) and breast (MDA-MB-231) human cancer cell lines, as well as non-cancerous HUVEC cell line.The ARHS extract displayed dose-dependent cytotoxic effects on HuH-7 and MDA-MB-231 cells, with respective IC 50 values of 2.39 and 5.67 µg/mL.Additionally, the ARHS extract impeded the migration of HuH-7 cells in a time-dependent fashion.Morphological assessments showed characteristics indicative of apoptosis in the treated cells.ARHS extract induced ROS production in HuH-7 cells.This study revealed that the A. rosea hexane extract effectively inhibited HuH-7 cell growth by inducing apoptosis, as evidenced by the changes in cell and nuclear morphology observed using DAPI and AO/EBr staining.Heptadeca-8,11-dien-1-yl demonstrated the highest docking score, forming three hydrogen bonds with the amino acids ARG-842A, ALA-1050A, and ASP-1052A in the 4ASD receptor.Although it has potential as an anticancer agent, additional research is needed to explore the in vivo anticancer efficacy.
Institution: King Saud University
Language: English
Topic: Medicinal Plant Research
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4406437949