Work

Cryolysate of Tumor Cells: From Technology of Its Obtaining Up to Creation of Anti-Cancer Vaccines

Year: 2024

Type: article

Abstract: The possibility of obtaining immune dendritic cells (iDCs) from bone marrow mononuclear cells has been proven by adding granulocyte-macrophage colony-stimulating factor, interleukin-4, and tumor cell cryolysate to the culture as an inducer of their maturation. The source of tumor antigens for obtaining iDCs was a cryolysate of Ehrlich adenocarcinoma cells, which was obtained by 5-fold freeze-thawing. It was established that the differentiation of mononuclear cells into iDCs is accompanied by the loss of the CD14 antigen, the appearance of maturity marker characteristics of these cells (CD83, CD11c antigens) and an increase in the expression of costimulatory molecules (CD80, CD86). The research results enable not only to evaluate the qualitative characteristics of iDCs obtained thereby, but also to outline the prospects of their use in immune correction of oncopathology.

Source: Problems of Cryobiology and Cryomedicine

DOI: 10.15407/cryo34.03.226

Authors: Anatoliy Goltsev, Yulia Gaevska, Tetyana Dubrava, Mykola Bondarovych, Olena Lutsenko, Maksym Ostankov

Institution: Institute for Problems of Cryobiology and Cryomedicine

Language: English

Topic: Immunotherapy and Immune Responses

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W4406443824

FWCI: 0.278

Cites: 18

Cited by: 2

Related to: 10