Assays to enhance metabolic phenotyping in the kidney
Year: 2025
Type: article
Abstract: We show significant metabolic differences between both primary and immortalized cells, and among primary cells with different durations in cell culture. In addition, 3 H-palmitate oxidation in tissue ex vivo is described as a method novel to the kidney for assessing the complete oxidation of long-chain fatty acids. This method shows that female mice have significantly increased fatty acid oxidation across two different strains of mice and significant strain-specific effects on metabolism.
Authors: Safaa Hammoud, Justin Kern, Sandip Mukherjee, Andrew J. Lutkewitte, Prabhleen Singh, Kate Newberry, Brian N. Finck, Leslie S. Gewin
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: bronze
Awards: G1271631115, G151374072, Blocking tubular cell cycle induces protective metabolic changes in chronic kidney disease, G193963534, Renal Oxygenation and Mitochondrial Function in AKI, G2337220457, G4586587292, G4886927091, G5465298251, G5788719373, G6639498476, G6657735289, G7671392149, G7930317202, G9000025623, G9000078046, G9000081704, G9000124636, G9000153645, G9000251191, G9000258209, Cell Cycle and Metabolism in Chronically Injured Renal Tubules
OpenAlex ID: W4406499003