Influence of Dust Load Intensity on Immune Factors in Coal Mine Workers with Chronic Obstructive Pulmonary Disease
Year: 2024
Type: article
Study design: Observational Study
Abstract: Material and methods. Markers of mature T-lymphocytes (CD3), T-helpers (CD4), T-cytotoxic cells (CD8), normal killer cells (CD16) and B-lymphocytes (CD22) in peripheral blood were detected using monoclonal antibodies in the direct immunofluorescence assay. The concentrations of interleukins -1, -4, -6, -8 and -10 were determined in the blood serum of miners by the enzyme immunoassay method using the respective test systems. Results. It was found that COPD in miners is combined with cellular immunity disorders (decrease in the percentage of CD3+ and CD4+ lymphocytes, increase in CD22+ cells), and an increase in serum concentrations of IL-1β, IL-4, IL-6, IL-8, IL-10 (p 0.05). Conclusion. It is shown that immune disorders depend on the degree of dust pollution in the workplace, very high levels of dust load cause a more pronounced decrease in the percentage of CD3+ cells and an increase in serum levels of IL-4 and IL-8 (p 0.05). All of the above-mentioned changes are important to consider for the correct development of future measures for primary and secondary prevention of COPD in coal miners, as well as taking into account the dust levels of workplaces.
Authors: Dmitry O. Lastkov, Olga Yu. Nikolenko, Eduard A. Maylyan, Sergey V. Grishchenko, Alexander S. Prilutsky, Olga A. Trunova, Andrey E. Bagriy, Anna V. Tishchenko
Institution: Donetsk National Medical University
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
OpenAlex ID: W4406619201