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Ganoderma lucidum polysaccharides relieve High Glucose-induced human renal tubular epithelial cell damage

Year: 2024

Type: article

Abstract: Objective Ganoderma lucidum polysaccharides (GLP) have biological and pharmacological actions in mammals, but its mechanism on high glucose (HG)-triggered human renal tubular epithelial cell (RTEC) injury is still unclear. Methods HK-2 cells were divided into Control, HG, GLP-I (HG + 3mg/L GLP), GLP-II (HG+ 6mg/L GLP), GLP-III (HG + 12mg/L GLP), GLP-II+PMA group. Cell proliferation and apoptosis were monitored using CCK-8 and flow cytometry. MDA, SOD, and ROS levels were determined using kits. Western blot assessed protein expression. Results Cell viability, Bcl-2, and ROS level in HG-treated HK-2 cells were enhanced by GLP exposure, whereas apoptosis rate, Bax and p65 expression, and MDA and ROS level were reduced. PMA treatment might partly abolish the effects of GLP on HG-induced cell damage. Conclusion GLP prevents HG-caused human RTEC damage by inhibiting NF-κB signal.

Source: Acta Poloniae Pharmaceutica - Drug Research

DOI: 10.32383/appdr/194853

Authors: Xinyu Wu, Weizhi Chang, Miao Qu, Zihe Guan, Jianuo Li, Xiaoyang Hu

Institution: Heilongjiang University of Chinese Medicine

Language: English

Topic: Fungal Biology and Applications

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Heilongjiang University