Ethanolic Extract of Red Okra Pods Induces Aberrant Spindle Segregation and Apoptotic Cell Death by Disrupting the Wnt Signaling Pathway in Colon Cancer Cells.
Year: 2024
Type: article
Abstract: Background: ) pods (EEROP) in inducing apoptosis in colorectal cancer cells (SW480) by inhibiting the Wnt/β-catenin signaling pathway. Methods: of EEROP in SW480 cells was determined by treating the cells with varying doses of EEROP, ranging from 0 to 1000 µg/mL. Apoptosis assay and signaling pathway analysis were performed through immunofluorescence staining and Western Blotting on SW480 cells treated with 250 µg/mL of EEROP for 72 hours. Results: EEROP treatment induced apoptosis in SW480 cells, marked by elevated levels of active caspase-3 (P<0.001) and cleaved poly-(ADP-ribose) polymerase (PARP)-1. Moreover, it notably decreased β-catenin protein levels, resulting in an augmented occurrence of cells displaying abnormal spindle segregation during mitosis (P=0.04). Conclusion: EEROP treatment reduces β-catenin protein levels, promotes abnormal spindle apparatus segregation, and finally leads to apoptotic cell death in CRC cells.
Source: PubMed
Authors: Firli Rahmah Primula Dewi, Sri Puji Astuti Wahyuningsih, Vuanghao Lim, Lionel Lian Aun In, Alfiah Hayati
Institutions: Universitas Airlangga, Universiti Sains Malaysia, UCSI University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: Kanazawa University
OpenAlex ID: W4406735401