rpl-11.1 Knock-Down Disturbs Translation Machinery and Proteostasis in Caenorhabditis elegans
Year: 2024
Type: article
Abstract: An array of human chronic diseases, such as Alzheimer’s disease, Huntington’s disease, and Parkinson’s disease, are related to defects in cellular proteostasis and the formation of protein aggregates. Using Caenorhabditis elegans PP563, a model organism developed for studying proteostasis stress, we can elucidate the biological role of specific genes and proteins involved in translation and proteostasis, advancing our understanding of relevant pathologies and therapeutics. Here, we report the knockdown of rpl-11.1 in C. elegans leads to disturbance in protein translation and proteostasis pathways, including the Ubiquitin-Proteosome System (UPS) and selective autophagy. We confirmed the importance of rpl-11.1 in ensuring correct ribosome biogenesis and translation accuracy. We also demonstrated that both the UPS and selective autophagy are involved in the clearance of misfolded and aggregated proteins. A minor experiment in this study revealed the importance of rpl-11.1 in germline proliferation.
Authors: Zishuo Sam Li, Jocelyne Mills, Dennis Bonal, Callie Millette
Institution: Brown University
Language: English
Subfield: Aging
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4406990888